摘要：研究了碱解法、酶解法和碱-酶复合水解法从豆渣中提取膳食纤维的工艺，通过单因素实验和正交实验对影响提取效果的碱浓度、酶用量、水解温度和时间的不同水平进行了优选，并得出最佳工艺为碱-酶复合水解法，其工艺条件为酶用量22万U、55℃和60min,同时碱浓度0.6%、75℃和90min。
关键词：大豆膳食纤维 木瓜蛋白酶 碱解 酶解
Abstract: The paper introduced the preparation of the soybean dietary fibefrom the soybean reside by alkali- hydrolysis,enzyme-hydrolysis and compoundhydrolysis.
In this paper,we studied the effects of the temperature and time,the density of NaOH and enzyme on the result,and have gained the optimal preparing condition of soybean dietary fiber:In the enzyme reactive,the amount of enzyme is 220000U,the temperature is 55℃,the reactive time is 60min;In the alkali reactive,the amount of NaOH is 0.6%,the temperature is 75℃,the reactive time is 90min。
Key Words:soybean dietary fiber, papain , alkali-hydrolysis ,enzyme-hydrolysis
随着人们生活水平的提高，膳食结构发生了很大的变化。膳食纤维（Dietary Fiber）作为一种功能性食品基料，越来越受到广泛的关注[1-3]。膳食纤维主要指纤维素、半纤维素、果胶等难以被人体消化吸收的多糖碳水化合物和类木质素。一般分为不可溶性纤维和水溶性纤维。不可溶性纤维，主要指细胞壁的构成物，包括纤维素、半纤维素、木质素及壳聚糖等，其中木质素属于芳香族碳氢化合物，可使细胞壁保持一定的韧性，存在于禾谷类和豆类种子的外皮及植物的茎和叶中。水溶性纤维，主要指能溶于水的果胶、植物胶和海藻多糖等，是指植物细胞内贮存物质及分泌物。……
